Wound Healing Assay Service
A wound healing test is a laboratory technique used to study cell migration and cell-cell interactions. This is also called a scratch test because it is done by scratching the cell monolayer and periodically capturing images through a time-lapse microscope. It is especially a 2D cell migration method for semi-quantitative measurement of cell migration in cell sheets. This scratch can be produced by various methods, such as mechanical, thermal or chemical damage. The purpose of this scratch is to create a cell-free area, hoping to induce cell migration and close the gap. The scratch test is only applicable to cell types that migrate as a collective epithelial layer, and not to non-adherent cells. Specifically, this assay is not suitable for chemotaxis studies. CD BioSciences can provide you with professional wound healing assay service to meet the specific needs of different customers.
Overall solution
Wound healing assay service has an experimental team composed of researchers, and relying on the detection platform, it can provide cell scratch detection services for domestic scientific researchers.
Protocol
Plate cells of choice in growth medium into a live cell imaging dish or chamber slide. It is important to ensure that a monolayer is formed as clumps will provide inaccurate results due to an uneven cell density. Titrating the cells to determine the optimal plating density is necessary.
When the confluency of the cell is ideal, use a pipette tip to scratch a wound through the entire center of the well. As mentioned previously, this is where the potential inconsistency comes into play with this assay. If the scratch is made manually, it is important to make sure that the wound is visible on both side of the field of view and should be around 0.5 mm wide.
The cells can then be placed on a microscope with a relative objective of 20x.
Begin time-lapse microscopy and adjust the parameters according to the variety of cells studying. Fast-growing cells may require shorter time intervals to acquire more accurate cell speed.
Features
1. Wound healing assay simulates the process of cell migration in the body to a certain extent.
2. Wound healing assay is very suitable for studying cell migration caused by the interaction between cells and extracellular matrix (ECM).
3. Wound healing assay is compatible with microscope systems including live cell imaging, and can be used to analyze the interaction between cells
4. Wound healing assay is the simplest method in vitro to study cell migration.
Our service items
| Project name | Wound healing assay service |
|---|---|
| Our services |
CD BioSciences offers wound healing assay service to meet the specific needs of different customers. |
| Material Required |
According to your scientific research needs. |
| Deliverables |
Study report |
| Price | Inquiry |
Our advantages
- CD BioSciences will complete your project on time and efficiently. We have professional after-sales service: which has been established to provide customers with efficient, fast, practical and feasible solutions.
- The core R&D team of CD BioSciences has a multidisciplinary background, including medicine, pharmacy, biology, materials science and other R&D backgrounds. They have active innovative thinking, rich biological expertise and experimental research experience.
- CD BioSciences specializes in wound healing assay service and we are committed to contributing to more customers and we can provide all services related to cellular immunofluorescence assay service.
Why choose us?
CD BioSciences provides professional wound healing assay service to meet the needs of regular customers to determine hits on time and on budget. CD BioSciences relies on the world-class technical expertise, we provide customers with the best quality one-stop wound healing assay service, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more information, our scientists will tailor the most reasonable plan for your project.
