Custom Purification Service
The separation and purification of proteins is widely used in biochemical research applications and is an important operation technique. A typical eukaryotic cell can contain thousands of different proteins, some are very rich, and some contain only a few copies. In order to study a certain protein, the protein must first be purified from other proteins and non-protein molecules. Most of the protein produced requires additional polishing to obtain higher purity downstream applications. The technical team of CD BioSciences has extensive experience in optimizing processes for unstable and challenging proteins (such as bispecific antibodies, fusion proteins and non-antibody protein therapeutics). For more information, please contact our customer support team.
Principle
Protein purification must use the inherent similarities and differences between different proteins, use the similarities between various proteins to remove the pollution of non-protein substances, and use the differences of each protein to purify the target protein from other proteins. Each protein has differences in size, shape, charge, hydrophobicity, solubility, and biological activity. Using these differences, the protein can be extracted from a mixture such as E. coli lysate to obtain a recombinant protein.
Overall Solutions
• Endotoxin removal and testing services
As a kind of heat source, endotoxin can cause inflammation and fever in organisms. Therefore, when recombinant protein is used in certain animal experiments, endotoxin removal is required to avoid adverse reactions in animals and affect experimental results. CD BioSciences endotoxin removal and detection service is an important service item of prokaryotic protein expression and purification service.
• Soluble prokaryotic protein expression
CD BioSciences' prokaryotic protein expression service uses the E. coli expression system for recombinant protein production. Customers only need to provide the gene or protein sequence corresponding to the target protein and provide relevant requirements at the same time. We will deliver in accordance with the amount of expression and purity agreed in the contract.
• Antibody purification
CD BioSciences provides antibody preparation, antibody purification and other-stop services, including gene sequencing, recombinant antibody expression, antibody purification to affinity determination, etc. You only need to provide the antibody or antiserum to be purified, and we will finally deliver antibodies with at least >90% purity.
• Large-scale production of protein fermentation
CD BioSciences has a mature protein expression system and advanced protein expression technology. We provide large-scale recombinant protein preparation services including: protein fermentation, high-density fermentation technology and process optimization.
• Tag-free protein purification
CD BioSciences provides guaranteed label-free protein purification services (an important component of prokaryotic protein expression services). Tag-free protein purification has the same sequence information as the natural protein, which is more conducive to protein crystallization experiments and protein function research.
• Inclusion body protein refolding service
The technical service team of CD BioSciences has many years of experience in protein renaturation, and can obtain large amounts of high-purity, stable and soluble recombinant proteins (such as enzyme preparations, cytokines, etc.) from the E. coli expression system.
Our Advantages
- CD BioSciences flexibly and comprehensively provide all services related to custom purification service.
- Our research supervisor and project manager will complete your project on time through open communication channels and meet your requirements.
- CD BioSciences has established a professional after-sales service team to provide customers with efficient and practical solutions.
Our Service Items
| Project name | Custom purification service |
|---|---|
| Our services |
✓ Endotoxin removal and testing services ✓ Soluble prokaryotic protein expression ✓ Antibody purification ✓ Large-scale production of protein fermentation ✓ Tag-free protein purification ✓ Inclusion body protein refolding service |
| Product delivery mode |
We will complete the project on time according to the agreed time. CD BioSciences offer you the following results: 1. All purified protein 2. Study report 3. COA including CE-SDS analysis |
| Price | Inquiry |
Why choose us?
CD BioSciences provides professional custom purification services to meet the needs of regular customers to determine hits on time and on budget. CD BioSciences relies on the world-class protein expression platform and technical expertise, we provide customers with the best quality one-stop custom purification services, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.
