AAV Serotype Screening Service

Adeno-associated virus vector is a vector that can be artificially transgenic, which is produced after genetic engineering using certain characteristics of naturally-occurring adeno-associated virus. According to the difference of capsid protein, AAV can be divided into 12 serotypes (AAV-1~AAV-12) and more than 100 variants. Different AAVs have different capsid protein spatial structures, sequences and tissue specificities, so the cell surface receptors they recognize and bind to are also correspondingly different. This also leads to different serotypes of transfected cell types and infection efficiency. Each is different, so how to choose the right serotype is very important. CD BioSciences offers AAV serotype screening service to meet the specific needs of different customers.

Overall solutions

Adeno-associated virus (AAV) has been widely used in the field of basic scientific research and gene therapy due to its characteristics of good diffusion, strong specificity, high safety, good stability and low immunogenicity. However, in research, researchers often encounter a problem, that is, it is impossible to confirm which AAV serotype is more suitable for the target tissue/cell under study. To solve this problem, CD BioSciences provides professional AAV serotype screening services, which can help scientific researchers quickly screen out the ideal serotype suitable for their experiments.

For the AAV serotype screening service, customers need to provide the following information:

• 1. The client completes the observation part of the pre-viral infection/injection and the positive result. CD BioSciences assists the client to complete the ideal AAV serotype screening. Customers need to provide ≥4ug total RNA, or ≥10E6 cell samples, or ≥300mg fresh tissue samples in the form of dry ice.
• 2. CD BioSciences provides a complete in vitro AAV serotype screening service. Customers need to provide pre-used AAV serotype mix.

Table 1. The affinity of different serotypes of AAV in various tissues and organs

AAV1	Muscle, heart, skeletal muscle (including myocardium), nerve tissue
AAV2	Central nervous system, muscles, liver, brain tissue, eyes
AAV5	Lungs, eyes, central nervous system, joint synovium, pancreas
AAV6	Lungs, heart
AAV7	Muscle, liver
AAV8	Liver, eyes, central nervous system, muscles

Our service items

Project name	AAV serotype screening service
Our service process	Customers provide needs Infection Cells or tissue sections preparation and Immunostaining Observing fluorescence via fluorescence microscope RNA Extraction andreverse transcription The qPCR method is used todetect different serotypeAAV infection ability Caculation
Product delivery mode	We will complete the project on time according to the agreed time, summarize the experimental data and conclusions, and complete the experimental report.
Price	Inquiry

Why choose us?

CD BioSciences provides corresponding AAV serotype screening service. AAV serotype screening service is a personalized and customized innovative scientific research service. Before determining the corresponding analysis plan and price, each project needs to be evaluated. Our experienced and dedicated scientific researchers ensure that the most suitable methods and techniques are selected for each adeno-associated virus project. If you want to know more about service prices or technical details, please feel free to contact us.